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End repair, A-tailing, and adapter ligation were performed enzymatically, followed by PCR amplification with Herculase II Fusion DNA Polymerase (814 cycles depending on input quality)

Recommended Resources JAX Therapeutic Antibody Evaluation Services Blog: Quick Facts to Improve Antibody Half-Life Measurements Blog: Revealing Antibody Half-Life and Stability Using FcRn Mouse Models from JAX Webinar: De-Risk Therapeutic Antibody Drug Development Using Humanized FcRn Mice References Betts A, Keunecke A, van Steeg TJ, van der Graaf PH, Avery LB, Jones H, Berkhout J

Nitroglycerin (GTN) is used for angina pectoris to relieve chest pain, but must be avoided in hypotensive patients