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Strikingly, we found acinar cells were replaced by SOX2 + cells in the IR SLG (GFP + cells) similar to the nonIR control with the production of both SOX2 + GFP + and SOX2negative progeny (Fig 5D, white arrowheads indicate SOX2negative progeny), suggestive of the presence of a transitamplifying cell which is no longer SOX2 + but derived from a SOX2 + cell (i.e., lineagetraced)

A better long term plan is to improve sleep hygiene, support balanced nutrition, manage stress, and use targeted supplements only where genuine deficiencies or lifestyle gaps exist

The models were refined using a combination of manual adjustments in COOT (version 0.9.8.7) 46 , automated real-space refinement in PHENIX (version 1.21) 47 and semi-automated refinement using ISOLDE (version 1.7.1) in ChimeraX (version 1.7) 48,49,50