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Sample concentrations were interpolated from the standard curve using a four-parameter logistic fit and adjusted for dilution, with intra-assay CVs maintained below 10% and recoveries ranging from 85 to 115%

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Along this line we observed that while exposure of the cells to 2 M sterosproine (STS), induced the cleavage of PARP that is a hallmark of apoptosis, R4VPL3-1 did not cause cleavage of PARP (Fig

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5.1.2 Thioredoxin reductase Thioredoxin reductase (TrxR) is a homodimeric enzyme that boasts a sophisticated architecture, featuring a flavin adenine dinucleotide (FAD) and a reduced nicotinamide adenine dinucleotide phosphate (NADPH) binding site for each of its monomers, thereby facilitating its essential role in cellular redox homeostasis (183)

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