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To characterize startle mediated astrocytic activation in the two groups of mice, mice were subjected to 10 air puffs delivered at 10 Hz directed to the vibrissa, nasal and facial region contralaterally to the recording side once per trial at 150 s in a 300 s two-photon imaging recording

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The targeted frequencies and specific areas of the brain are specific to conditions or dysregulation we are trying to address during neurofeedback therapy