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MDC assays To evaluate GPX4 serotonylation, GPX4 (10 g) and TGM2 (0.25 g) proteins were added to an enzymatic buffer (25 mM Tris-Cl (pH 8) and 5 mM CaCl 2 plus protease inhibitors), followed by the addition of MDC (SigmaAldrich, D4008, 5 mM) or 5-HT (5 mM)

doi: 10.31857/S0026898423060228 166 ZhaiC.-Z.ZhaoL.YinL.-J.ChenM.WangQ.-Y.LiL.-C.et al

Sam Rhee: And, uh, now that you've taken it for a month, [00:15:00] would you say you should, it works fine as it is, or should it be integrated with other types of therapies or wellness treatments

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Finally, the specific molecules identified here may represent interesting therapeutic candidates