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A clinical trial of the effects of dietary patterns on blood pressure

To label nuclei, hair follicles were incubated for 30 to 45 min with Hoechst 33258 (life Technologies, stock at 10 mg/mL) diluted 2000x in supplemented Williams medium

(PubMed) Buckley MS, Goff AD, Knapp WE

1 H-NMR(D 2 O, ppm): 2.22.3(m, 2H, CH 2 CO 2 NBu 4 ), 2.52.8(m, 2H), 3.23.3(m, 1H, 3-H) 13 C-NMR(D 2 O, ppm): 41.09(CH 2 CO 2 NBu 4 ), 48.23(4-CH 2 ), 51.14(3-CH), 178.54(CO 2 NBu 4 ) Triethylamine (790 mg, 7.81 mmol) was added to the reacting solution of D 2 O containing (S)-4-hydroxy-3-methanesulfonyl hydroxybutyric acid, so prepared from Example 3 and stirred at room temperature for 30 minutes

As expected, at the end of the experiment, HFD mice gained 20% more BW than STD mice (Supplementary Figure S1A), together with increased heart weight (HW) and serum NEFA (Supplementary Figures S1BD)

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