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13 C-labeling treatment and GC-MS analysis The cells of the WT and ldhA OE transformed strains in the logarithmic growth phase were collected by centrifugation, washed with f/2 medium devoid of any carbon source, and inoculated into fresh f/2 medium containing either 5 mM sodium lactate (containing 30% 13 C-lactate + 70% 12 C-lactate) and 0.174 gL 1 sodium bicarbonate or 0.174 gL 1 13 C-sodium bicarbonate and 5 mM 12 C-lactate medium, with the same initial cell number (18.72 10 6 cellsmL 1 ) and three biological replicates per treatment

Ted Hackstadt, NIH/NIAID Rocky Mountain Laboratories), or antibody specific for Rickettsia japonica OMP (Invitrogen, PA5-117668)

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Feng Y, Xu J, Shi M, Liu R, Zhao L, Chen X, et al

doi:10.1074/jbc.M116.752469

Glutathione plays a vital role in the body's natural detoxification pathways, helping to neutralize and eliminate harmful toxins and free radicals