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(2004) used a transgenic mouse model expressing the autophagosomal marker green fluorescent protein-microtubule associated protein 1 light chain 3 (GFP-LC3) to demonstrate that autophagy is significantly upregulated upon starvation in liver tissue but not in the brain

The expression levels of GPX4 were determined by Western blotting, measuring the grayscale ratios of the GPX4 band to the GAPDH band in each group, with the ratio in the Control group normalized to 1

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