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In addition to the indirect consequences of long-term oral antibiotic consumption on intestinal barrier health, particularly through reducing the numbers of SCFA-producing species, these therapeutics have also been reported to directly impair the gut barrier by reducing the expression of TJ proteins and negatively impacting their morphology

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Increased fecal microbiota diversity and altered composition Fecal microbiome diversity (-diversity), as assessed by 16S sequencing, increased by W12 (p=0.021) and remained higher than baseline at subsequent W24 and W52 timepoints (Fig

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Figure 4 3.4 Confirmation of essentiality of genes and immunogenicity of knockouts in calf trial 3.4.1 Bacteria shedding in fecal matter The MAP culture qPCR indicated that all the inoculated animals had passive MAP shedding 24 hours after inoculation