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AGC target value for fragment spectra was set at 1E5 with a resolution of 15,000 and injection times of 22 ms and Top12

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Measurements from the 1966 study had shown elevated chloride concentrations in the Flint River, still true in 2014

Flow cytometry was performed on the DPCs, and quantification was done using Image J Pro software

Besides, to further confirm peroxynitrite-mediated activation of the NF-B pathway, we also measured the mRNA expression levels of known NF-B p65 target genes Bax , P53 , Bcl2 , and Bcl-xL