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For preparing the parasite cell extract, pellets were diluted in equal volumes of phosphate-buffered saline, and a complete protease inhibitor cocktail (Roche, Mannheim, Germany) was added

doi: 10.1155/2019/6793596 105 CrosnierCHokkeCHProtasioAVBrandtCRinaldiGLangenbergMCCet al

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A 2025 Nature Communications paper mapped the binding to the disordered transactivation domain of p53, adding structural detail to how FOXO4-DRI engages its target